Replaces Prod. #: BML-P410
Highly specific, potent and reversible inhibitor of caspase-3. Ki<1nM for purified caspase-3. IC50=0.2nM for PARP cleavage in cultured human osteosarcoma cell extracts. In contrast, the caspase-1 (ICE) specific inhibitor YVAD-CHO has a Ki=12µM for caspase-3 and IC50>10µM for PARP cleavage in osteosarcoma cell extracts. The DEVD sequence is based on PARP cleavage site Asp-216 for caspase-3.
Product Details
| Alternative Name: | Caspase-3 inhibitor (aldehyde), Caspase-7 inhibitor (aldehyde) |
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| Sequence: | Ac-Asp-Glu-Val-Asp-CHO |
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| Formula: | C20H30N4O11 |
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| MW: | 502.5 |
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| CAS: | 169332-60-9 |
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| Formulation: | Lyophilized. |
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| Peptide Content: | 70-90%. |
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| Purity: | ≥95% (HPLC) |
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| Solubility: | Soluble in distilled water or DMSO. |
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| Reconstitution: | Add 0.2ml DMSO per 1mg peptide. Shake well until all the contents are dissolved. This furnishes a 10mM solution of this material. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stock solutions should be divided into aliquots and stored at -20°C. Solutions are stable for at least 1 month when stored at -20°C in the dark and protected from moisture. |
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| Handling: | Keep under nitrogen. Keep cool and dry. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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The Therapeutic Value of SC66 in Human Renal Cell Carcinoma Cells: M. Xu, et al.; Cell Death Dis.
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Curcumin differentially affects cell cycle and cell death in acute and chronic myeloid leukemia cells: M. Martinez-Castillo, et al.; Oncol. Lett.
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Caspase-6 Undergoes a Distinct Helix-Strand Interconversion upon Substrate Binding: K.B. Dagbay, et al.; J. Biol. Chem.
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Full Text
Albumin-binding caspase-cleavable prodrug that is selectively activated in radiation exposed local tumor: S.W. Chung, et al.; Biomaterials
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Application(s): Cell culture,
Abstract;
Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts: R. Ghosh, et al.; Int. J. Cardiol.
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Application(s): Measurement of Caspase 3 activity - mouse heart, liver, and kidney,
Abstract;
MicroRNAs in Hyperglycemia Induced Endothelial Cell Dysfunction: M. Silambarasan, et al.; Int. J. Mol. Sci.
17, 518 (2016),
Application(s): Cell culture,
Abstract;
Full Text
Differential apoptosis-related protein expression, mitochondrial properties, proteolytic enzyme activity, and DNA fragmentation between skeletal muscles: E.M. McMillan, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
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Full Text
Inhibition of ubiquitin-proteasome pathway activates a caspase-3-like protease and induces Bcl-2 cleavage in human M-07e leukaemic cells: X.M. Zhang, et al.; Biochem. J.
340, 127 (1999),
Abstract;
Full Text
Interferon gamma induces upregulation and activation of caspases 1, 3, and 8 to produce apoptosis in human erythroid progenitor cells: C. Dai & S.B. Krantz; Blood
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Abstract;
Inhibition of human caspases by peptide-based and macromolecular inhibitors: M. Garcia-Calvo, et al.; J. Biol. Chem.
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Abstract;
Full Text
A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro: Z. Han, et al.; J. Biol. Chem.
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Abstract;
Full Text
CPP32/apopain is a key interleukin 1 beta converting enzyme-like protease involved in Fas-mediated apoptosis: J. Schlegel, et al.; J. Biol. Chem.
271, 1841 (1996),
Abstract;
Full Text
ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis: D.W. Nicholson; Nature Biotechnol.
14, 297 (1996),
Abstract;
Sequential activation of ICE-like and CPP32-like proteases during Fas- mediated apoptosis: M. Enari, et al.; Nature
380, 723 (1996),
Abstract;
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature
376, 37 (1995),
Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik et al.; Nature
371, 346 (1994),
Abstract;
