[pSer²⁶²]Tau polyclonal antibody



ALX-215-001-R100	100 µl	581.00 USD
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Product Details

Alternative Name:	Neurofibrillary tangle protein

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to the region of human tau containing phosphorylated Ser²⁶².

UniProt ID:	P10636

Species reactivity:	Human

Crossreactivity:	Expected to react with mouse, rat, goat, monkey, bovine and baboon tau.

Applications:	WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Epitope affinity-purified. Negatively preadsorbed by non-phosphorylated immunogen peptide.

Formulation:	Liquid. In Dulbecco's PBS, pH 7.3, 1mg/ml BSA and 0.05% sodium azide.

Handling:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Shipping:	Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Scientific Background:	Tau phosphorylation at Ser202 and Ser262 is important for the regulation of microtubuli assembly.

Technical Info/Product Notes:	Replacement for ADI-KAP-MA306

Regulatory Status:	RUO - Research Use Only

Figure: Peptide competition. Only the phosphopetide corresponding to tau [pS²⁶²] blocks the antibody signal, demonstrating the specificity of the antibody. Tau [pS²⁶²] phophorylation is induced by the addition of PKA.Method: Human recombinant tau untreated (lane 1) or treated with PKA (36μg per μg tau) for 45 min and added to background extracts was resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, then incubated with PAb to Tau (phosphorylated) (pSer262) (Prod. No. ALX-215-001) for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab’)₂ anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal^TM method.

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Product Literature References

Dephosphorylation of tau by protein phosphatase 5: impairment in Alzheimer's disease: F. Liu, et al.; J. Biol. Chem. 280, (2005), Abstract; Full Text

Involvement of aberrant glycosylation in phosphorylation of tau by cdk5 and GSK-3beta: F. Liu, et al.; FEBS Lett. 530, 209 (2002), Abstract;

Role of glycosylation in hyperphosphorylation of tau in Alzheimer’s disease: F. Liu, et al.; FEBS Lett. 512, 101 (2002), Abstract;