Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

TRAM (human) polyclonal antibody (AL239)

ALX-210-914-R050 50 µl 158.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:TRIF-related adaptor molecule, TIRP, Toll-Interleukin 1 receptor [TIR] domain-containing protein, TICAM-2, TICAM 2, TIR-containing adaptor molecule-2
Immunogen:Synthetic peptide corresponding to aa 2-29 (G2IGKSKINSCPLSLSWGKRHSVDTSPGY29) of human TRAM (TRIF-related adaptor molecule).
UniProt ID:Q86XR7
Species reactivity:Human
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. Neat serum.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
TRAM (human) polyclonal antibody (AL239) Western blot
Figure: Detection of TRAM (human) in HEK 293T transfected cells by the PAb to TRAM (human) (AL239) (Prod. No. ALX-210-914).Method: 10µg per lane of total cell extracts from mock transfected HEK 293T cells (lane 1) or HEK 293T cells transfected with an expression vector encoding VSV-tagged human TRAM (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer to nitrocellulose, the membrane was probed with AL239 at a 1:1’000 dilution. After 1 hour of incubation at room temperature, the membrane was extensively washed and then incubated with goat anti-rabbit horseradish peroxydase (1:4’000 dilution; Jackson, Prod. No. 111-035-144) for ECL detection. Note: In the mock transfected HEK293T cells, a band corresponding to endogenous TRAM is detected."
Please mouse over
TRAM (human) polyclonal antibody (AL239) Western blot

Product Literature References

Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells: E. Aksoy, et al.; Blood 109, 2887 (2007), Abstract; Full Text

General Literature References

TIRP, a novel Toll/interleukin-1 receptor (TIR) domain-containing adapter protein involved in TIR signaling: L.H. Bin, et al.; J. Biol. Chem. 278, 24526 (2003), Abstract; Full Text
TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway: M. Yamamoto, et al.; Nat. Immunol. 4, 1144 (2003), Abstract; Full Text

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
TICAM Polyclonal antibody
By catalog section:


Technical Service
Customer Service