Product Specification
| Alternative Name: | TRIF-related adaptor molecule, TIRP, Toll-Interleukin 1 receptor [TIR] domain-containing protein, TICAM-2, TICAM 2, TIR-containing adaptor molecule-2 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 2-29 (G2IGKSKINSCPLSLSWGKRHSVDTSPGY29) of human TRAM (TRIF-related adaptor molecule). |
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| UniProt ID: | Q86XR7 |
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| Species reactivity: | Human
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. Neat serum. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Detection of TRAM (human) in HEK 293T transfected cells by the PAb to TRAM (human) (AL239) (Prod. No.
ALX-210-914).
Method: 10µg per lane of total cell extracts from mock transfected HEK 293T cells (lane 1) or HEK 293T cells transfected with an expression vector encoding VSV-tagged human TRAM (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer to nitrocellulose, the membrane was probed with AL239 at a 1:1’000 dilution. After 1 hour of incubation at room temperature, the membrane was extensively washed and then incubated with goat anti-rabbit horseradish peroxydase (1:4’000 dilution; Jackson, Prod. No. 111-035-144) for ECL detection.
Note: In the mock transfected HEK293T cells, a band corresponding to endogenous TRAM is detected."
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Product Literature References
Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells: E. Aksoy, et al.; Blood
109, 2887 (2007),
Abstract;
Full Text
General Literature References
TIRP, a novel Toll/interleukin-1 receptor (TIR) domain-containing adapter protein involved in TIR signaling: L.H. Bin, et al.; J. Biol. Chem.
278, 24526 (2003),
Abstract;
Full Text
TRAM is specifically involved in the Toll-like receptor 4-mediated MyD88-independent signaling pathway: M. Yamamoto, et al.; Nat. Immunol.
4, 1144 (2003),
Abstract;
Full Text
