Product Details
| Alternative Name: | AdipoR1, CGI-45 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 2-36 (S2SHKGSVVAQGN
GAPASNREADTVELAELGPLLEE36) of human adiponectin receptor 1 (AdipoR1). |
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| UniProt ID: | Q96A54 |
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| Species reactivity: | Human
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 1:100)
Western blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Liquid. Neat serum. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Detection of Adiponectin Receptor 1 (human) in HEK 293T transfected cells by the PAb to Adiponectin Receptor 1 (human) (AL238) (Prod. No. ALX-210-913).Method: 10µg per lane of total cell extracts from mock transfected HEK 293T cells (lanes 1 and 3) or HEK 293T cells transfected with an expression vector encoding the Flag-tagged human AdipoR1 (lanes 2 and 4) were run on a 12% SDS-PAGE gel and analyzed by Western blot. Lanes 1 and 2 were probed with an anti-FLAG antibody at a 1:2’000 dilution. Lanes 3 and 4 were probed with AL238 at a 1:1’000 dilution. After 1 hour of incubation at RT, the membrane was extensively washed and then incubated with goat anti-mouse horseradish peroxydase (Prod. No. APO-26G-050H) or a goat anti-rabbit horseradish peroxydase (Jackson, Prod. No. 111-035-144), respectively, for ECL detection. Note that the upper bands might represent oligomerized forms of the receptor.
Figure 2: Paraffin embedded human adipose tissue stained with PAb to Adiponectin Receptor 1 (human) (AL238) (Prod. No. ALX-210-913).
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General Literature References
Cloning of adiponectin receptors that mediate antidiabetic metabolic effects: T. Yamauchi, et al.; Nature
423, 762 (2003),
Abstract;
