Product Specification
| Host: | Rabbit |
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| Immunogen: | Recombinant human full-length caspase-9. |
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| UniProt ID: | P55211 |
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| Species reactivity: | Human
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| Specificity: | Recognizes procaspase-9 and the p37/p35 cleavage products of activated caspase-9. |
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| Applications: | IP, WB
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| Application Notes: | Recommended for the detection of endogenous caspase-9 activation. |
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| Formulation: | Liquid. Antiserum containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: (A) Schematic structure of caspase-9 and its cleavage products as detected by anti-caspase-9.
Figure: (B) Detection of caspase-9 processing during apoptosis. The antiserum detects procaspase-9 (46kDa) and the intermediate cleavage products of 37kDa and 35 kDa. No cross-reactivity with other caspases is observed.
Method: Jurkat cells were treated with 2µM staurosporine (Prod. No.
ALX-380-014). After 4 hours cell lysates were prepared and separated by SDS-Page (2x10
6 cells/lane) under reducing conditions. Proteins were immunoblotted with rabbit anti-caspase-9 (1:1’000). Following incubation with peroxidase-conjugated secondary antibodies caspase-9 processing was detected by enhanced chemoluminescent staining. The closed arrow indicates procaspase-9 (46kDa), which processed to the p35 and p37 fragments (open arrows)."
Figure: (C) Immunoprecipitation of caspase-9.
Method: Extracts of 1x107 Jurkat cells were subjected to immunoprecipitation with Protein G-sepharose in the presence of rabbit preimmune serum (PI control, lane 1) or the antiserum to caspase-9 (lane 2). Lane 3 demonstrates that the antiserum is able to completely deplete caspase-9 from cell lysates, as no caspase-9 was detected in the supernatants after precipititaion.
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Product Literature References
The prevention of spontaneous apoptosis of follicular lymphoma B cells by a follicular dendritic cell line: involvement of caspase-3, caspase-8 and c-FLIP: J.J. Goval, et al.; Haematologica
93, 1169 (2008),
Application(s): WB using human follicular lymphoma lysate,
Abstract;
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