Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Caspase-1 polyclonal antibody

ALX-210-804-C100 100 µg 435.00 USD
Do you need bulk/larger quantities?

Product Details

Alternative Name:IL-1β converting enzyme, Interleukin-1β converting enzyme, ICE
Immunogen:Synthetic peptide corresponding to aa 224-242 (CEPH224KTSDSTFLVFMSHGIREG242) of human caspase-1.
UniProt ID:P29466
Species reactivity:Human, Mouse, Rat
Applications:ICC, IHC, IP, WB
Recommended Dilutions/Conditions:Immunocytochemistry (10-20µg/ml)
Immunohistochemistry (1:50)
Immunoprecipitation (4-8µg/ml)
Western Blot (0.5-4µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands of ~45kDa (procaspase-1) and ~20kDa (cleavage product) by Western blot.
Purity Detail:Epitope-affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.5mM EDTA, and 0.03% Proclin.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
Caspase-1 polyclonal antibody Western blot
Figure: Western blot analysis of caspase-1 expression in rat liver tissue lysate using Caspase-1, pAb (Prod. No. ALX-210-804)."
Caspase-1 polyclonal antibody Immunohistochemistry
Immunohistochemistry staining of human lung tumor tissue using Caspase-1, pAb (Prod. no. ALX-210-804) at a 1:50 dilution showed positive staining in both cytoplasm and nucleus of lung epithelial cells. Images were taken at magnification of 20 x (A) and 63 x (B).
Please mouse over
Caspase-1 polyclonal antibody Western blot Caspase-1 polyclonal antibody Immunohistochemistry

Product Literature References

Protein-Bound Uremic Toxins Induce Reactive Oxygen Species-Dependent and Inflammasome-Mediated IL-1β Production in Kidney Proximal Tubule Cells: M. Mihajlovic, et al.; Biomedicines 9, 1326 (2021), Abstract;
FANCA and FANCC modulate TLR and p38 MAPK-dependent expression of IL-1β in macrophages: M.R. Garbati, et al.; Blood 122, 3197 (2013), Abstract;