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8-Hydroxyguanosine polyclonal antibody

ALX-210-802-R100 100 µl 276.00 USD
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Product Details

Immunogen:Synthetic 8-hydroxyguanosine-conjugate.
Species reactivity:Species independent
Specificity:Recognizes 8-hydroxyguanosine and 8-hydroxy-2'-deoxyguanosine.
Crossreactivity:Partially cross-reacts with 8-mercaptoguanosine and slightly with 8-bromoguanosine. Does not cross-react with other naturally occuring nucleotides.
Applications:ELISA, IHC (FS), IHC (PS)
Recommended Dilutions/Conditions:ELISA (1:250,000)
Immunohistochemistry (paraffin sections, 1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. Neat serum.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging and neurodegenerative conditions, such as Alzheimer's disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.
Regulatory Status:RUO - Research Use Only

Product Literature References

Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise: S. Golbidi, et al.; Br. J. Pharmacol. 171, 5345 (2014), Application(s): IHC on FFPE tissue sections, Abstract; Full Text
Effects of HIF-1alpha and HIF2alpha on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts: S. Biswas, et al.; J. Oncol. 2010, 757908 (2010), Abstract; Full Text
Decreased blood pressure in NOX1-deficient mice: G. Gavazzi, et al.; FEBS Lett. 580, 497 (2006), Abstract;

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