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CCR8 (human) polyclonal antibody



ALX-210-764-C100	100 µg	311.00 USD
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Product Specification

Alternative Name:	C-C chemokine receptor 8, CD198

Host:	Goat

Immunogen:	Synthetic peptide corresponding to aa 184-193 (Y¹⁸⁴SFYNQQTLK¹⁹³) of the 2^nd extracellular domain of human CCR8 (C-C Chemokine Receptor 8).

UniProt ID:	P51685

Species reactivity:	Human

Applications:	ICC, IHC (PS), WB

Recommended Dilutions/Conditions:	Immunocytochemistry (acetone fixed cells (1:800)) Immunohistochemistry (paraffin sections (1:300)) Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Epitope-affinity purified IgG.

Quality Control:	Immunocytochemistry on human K562 cells.

Formulation:	Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.

Handling:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Shipping:	Shipped on Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Protocol:	Immunohistochemistry on paraffin sections: - Fix paraffin sections in 10% formalin. - De-wax sections and block endogenous peroxide by incubating sections with 3% H₂O₂ for 15 minutes at room temperature (RT). -Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block the non-specific binding sites. - Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 minutes at RT. - Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 minutes. - Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 minutes. - Wash slides and incubate with DAB for 5-7 minutes at RT. - Wash slides with PBS and counter stain with hematoxylin.

Product Literature References

Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL: N.E. Annels, et al.; Blood 103, 2806 (2004), Abstract;

Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes: F. Annunziato, et al.; J. Exp. Med. 196, 379 (2002), Abstract; Full Text

The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque, et al.; Blood 97, 39 (2001), Abstract; Full Text

CC Chemokine I-309 is the Principal Monocyte Chemoattractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque, et al.; Circulation 102, 786 (2000), Abstract; Full Text

The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem. 273, 386 (1998), Abstract; Full Text

Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol. 26, 3021 (1996), Abstract;