Product Specification
Alternative Name: | C-C chemokine receptor 8, CD198 |
|
Host: | Goat |
|
Immunogen: | Synthetic peptide corresponding to aa 184-193 (Y184SFYNQQTLK193) of the 2nd extracellular domain of human CCR8 (C-C Chemokine Receptor 8). |
|
UniProt ID: | P51685 |
|
Species reactivity: | Human
|
|
Applications: | ICC, IHC (PS), WB
|
|
Recommended Dilutions/Conditions: | Immunocytochemistry (acetone fixed cells (1:800)) Immunohistochemistry (paraffin sections (1:300)) Western Blot (1:2,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Epitope-affinity purified IgG. |
|
Quality Control: | Immunocytochemistry on human K562 cells. |
|
Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide. |
|
Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
|
Shipping: | Shipped on Blue Ice |
|
Short Term Storage: | +4°C |
|
Long Term Storage: | -20°C |
|
Protocol: | Immunohistochemistry on paraffin sections: - Fix paraffin sections in 10% formalin. - De-wax sections and block endogenous peroxide by incubating sections with 3% H2O2 for 15 minutes at room temperature (RT). -Wash sections with PBS and incubate with 2% normal rabbit serum at room temperature to block the non-specific binding sites. - Wash sections with PBS and incubate with primary antibody (anti-chemokine receptor) at recommended dilution for 60 minutes at RT. - Wash slides and incubate at RT with biotinylated rabbit anti-goat IgG H+L (Vector Labs) at 1:200 dilution for 30 minutes. - Wash slides and incubate with HRP-Streptavidin (Vector Labs) 1:400 dilution for 30 minutes. - Wash slides and incubate with DAB for 5-7 minutes at RT. - Wash slides with PBS and counter stain with hematoxylin. |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL: N.E. Annels, et al.; Blood
103, 2806 (2004),
Abstract;
Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes: F. Annunziato, et al.; J. Exp. Med.
196, 379 (2002),
Abstract;
Full Text
The chemokine receptor CCR8 mediates human endothelial cell chemotaxis induced by I-309 and Kaposi sarcoma herpesvirus-encoded vMIP-I and by lipoprotein(a)-stimulated endothelial cell conditioned medium: N.S. Haque, et al.; Blood
97, 39 (2001),
Abstract;
Full Text
CC Chemokine I-309 is the Principal Monocyte Chemoattractant Induced by Apolipoprotein(a) in Human Vascular Endothelial Cells: N.S. Haque, et al.; Circulation
102, 786 (2000),
Abstract;
Full Text
The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains: R. Horuk, et al.; J. Biol. Chem.
273, 386 (1998),
Abstract;
Full Text
Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor: M. Samson, et al.; Eur. J. Immunol.
26, 3021 (1996),
Abstract;