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Bcl-2 polyclonal antibody

ALX-210-701-C100 100 µg 443.00 USD
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Product Specification

Immunogen:Synthetic peptide corresponding to aa 20-34 of human Bcl-2.
UniProt ID:P10415
Species reactivity:Human, Mouse
Specificity:This antibody is particularly useful for the determination of Bcl-2 expression levels in species other than human.
Applications:IHC (FS), IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry:, Frozen sections: 5µg/ml; no pre-treatment needed.
Paraffin sections: 2.5µg/ml; pressure cooker antigen retrieval is recommended.
For both: DAB detection. Intestine or human lymph node can be used as positive control, brain tissue as negative control (only random cells will stain for Bcl-2 in brain tissue).
Western Blot: 5µg/ml; ECL detection. MCF-7 cells can be used as a positive control, normal rabbit IgG as negative control.
Titration is recommended for optimal results in individual systems.
Formulation:Liquid. In PBS containing 0.09% sodium azide.
Handling:Do not freeze.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C

Product Literature References

Eomes broadens the scope of CD8 T-cell memory by inhibiting apoptosis in cells of low affinity: I. Kavazovic, et al.; PLoS Biol. 18, e3000648 (2020), Abstract; Full Text

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