Product Specification
| Alternative Name: | MGMT |
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| Host: | Rabbit |
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| Immunogen: | Human MGMT (O6-methylguanine-DNA methyltransferase). |
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| UniProt ID: | P16455 |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1:100-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Formulation: | Contains 0.05% sodium azide. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | O6-Methylguanine-DNA methyltransferase (MGMT) removes O6-methylguanine from DNA by transferring the O6-alkyl group to a specific cysteine residue within its own sequence irreversibly inactivating MGMT. Unrepaired alkylated guanine will cause G·C -> A·T transition mutations by anomalous base pairing during DNA replication. DNA damage by O6-methylguanine can induce apoptosis regulated by Bcl-2 and caspases- 3 & -9. MGMT is able to remove O4-alkylated-thymine. |
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Product Literature References
O6-methylguanine-DNA methyltransferase activity in breast and brain tumors: I. Preuss, et al.; Int. J. Cancer
61, 321 (1995),
Abstract;
