Product Details
Alternative Name: | PBEF1, Pre-B-cell colony enhancing factor 1, Nicotinamide phosphoribosyltransferase |
|
Host: | Rabbit |
|
Immunogen: | Recombinant human Nampt (visfatin/PBEF). |
|
UniProt ID: | P43490 |
|
Species reactivity: | Human
|
|
Crossreactivity: | Weakly cross-reacts with mouse Nampt. |
|
Applications: | ELISA, WB
|
|
Recommended Dilutions/Conditions: | ELISA (direct and indirect, 1:2,000-1:5,000) Immunohistochemistry (paraffin sections, 1:500-1:1,000) Western Blot (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Purified IgG. |
|
Formulation: | Liquid. In PBS, pH 7.4. Contains no preservatives. |
|
Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
|
Shipping: | Blue Ice |
|
Long Term Storage: | -20°C |
|
Regulatory Status: | RUO - Research Use Only |
|
Figure: Western blot analysis of human Nampt using Nampt (Visfatin/PBEF) (human), pAb (Prod. No. ALX-210-425) at 1:2’000 dilution.Lane 1: Recombinant human Nampt (His tagged) (Prod. No.
ALX-201-319)Lane 2: LPS-treated human peripheral blood leukocyte lysate"
Please mouse over
Product Literature References
Visfatin, an adipocytokine with proinflammatory and immunomodulating properties: A.R. Moschen, et al.; J. Immunol.
178, 1748 (2007),
Abstract;
Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett.
580, 4105 (2006),
Abstract;
Related Products