Product Details
Alternative Name: | TLR3, CD283 |
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Host: | Rabbit |
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Immunogen: | Synthetic peptides corresponding to aa 135-150, 828-844 and 876-891 of mouse TLR3 (Toll-like receptor 3). |
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UniProt ID: | Q99MB1 |
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Species reactivity: | Mouse
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Crossreactivity: | Does not cross-react with human TLR3. |
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Applications: | Flow Cytometry, IHC (FS), IHC (PS), WB
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Recommended Dilutions/Conditions: | Flow Cytometry (cell surface & intracellular, 1µg/106 cells)
Immunohistochemistry (paraffin sections, 1:100; frozen sections, 1:20-1:1,000)
Western Blot (1-3µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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Positive Control: | Mouse spleen cell whole cell lysate can be used as positive control. |
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Purity Detail: | Protein G-affinity purified. |
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Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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Use/Stability: | Stable for 6 months when stored at +4°C. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Technical Info/Product Notes: | Replacement for ADI-CSA-790 |
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Regulatory Status: | RUO - Research Use Only |
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Intracellular flow analysis of TLR3 in Balb/c mouse splenocytes using PAb to Toll-like Receptor 3 (mouse) (Prod. No. ALX-210-367) at 1µg/106 cells. Shaded histogram represents cells without antibody; green represents rabbit IgG isotype control; red represents PAb to Toll-like Receptor 3 (mouse).
Western blot analysis of TLR3 in 15µg/lane mouse spleen tissue lysate using PAb to Toll-like Receptor 3 (mouse) (Prod. No. ALX-210-367).
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Product Literature References
Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice: P.S. Patole, et al.; Nephrol. Dial. Transplant.
21, 3062 (2006),
Abstract;
Toll-like receptor-7 modulates immune complex glomerulonephritis: R.D. Pawar, et al.; J. Am. Soc. Nephrol.
17, 141 (2006),
Abstract;
Viral double-stranded RNA aggravates lupus nephritis through Toll-like receptor 3 on glomerular mesangial cells and antigen-presenting cells: P.S. Patole, et al.; J. Am. Soc. Nephrol.
16, 1326 (2005),
Abstract;