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Osteocalcin (mouse) (NT) polyclonal antibody

ALX-210-333-C100 100 µg Inquire for pricing
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Product Details

Immunogen:Synthetic peptide corresponding to aa 1-20 (Y1LGASVPSPDP
LEPTREQCE20) of intracellular N-terminal mouse osteocalcin.
UniProt ID:P86547
Species reactivity:Mouse
Specificity:Recognizes intracellular N-terminal osteocalcin.
Crossreactivity:Does not cross-react with rat osteocalcin.
Applications:IHC (PS)
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 1-2µg/ml, after trypsin treatment)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Not suitable for WB.
Purity Detail:Protein A-affinity and epitope-affinity purified.
Formulation:Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA.
Reconstitution:Reconstitute with 50µl of Reconstitution Solution (water containing 0.1% sodium azide) to a final concentration of 2mg/ml. Further dilutions should be made using 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
Use/Stability:Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

Undercarboxylated, But Not Carboxylated, Osteocalcin Suppresses TNF-α-Induced Infammatory Signaling Pathway in Myoblasts: D. Park, et al.; J. Endocr. Soc. 6, bvac084 (2022), Abstract;
Circadian disruption by shifting the light-dark cycle negatively affects bone health in mice: M. Schilperoort, et al.; FASEB J. 34, 1052 (2019), Abstract; Full Text
Insulin-Like Growth Factor I Does Not Drive New Bone Formation in Experimental Arthritis: M.N. van Tok, et al.; PLoS One 11, e0163632 (2016), Application(s): Immunohistochemistry, ankle and tow section osteoblasts, Abstract; Full Text
Macrophages Promote Osteoblastic Differentiation In-Vivo: Implications in Fracture Repair and Bone Homeostasis: L. Vi, et al.; J. Bone Miner. Res. 30, 1090 (2015), Application(s):IHC on FFPE sections of mouse tibia, Abstract;

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