Product Details
Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 1-20 (Y1LGASVPSPDP LEPTREQCE20) of intracellular N-terminal mouse osteocalcin. |
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UniProt ID: | P86547 |
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Species reactivity: | Mouse
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Specificity: | Recognizes intracellular N-terminal osteocalcin. |
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Crossreactivity: | Does not cross-react with rat osteocalcin. |
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Applications: | IHC (PS)
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Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 1-2µg/ml, after trypsin treatment) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Not suitable for WB. |
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Purity Detail: | Protein A-affinity and epitope-affinity purified. |
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Formulation: | Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. |
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Reconstitution: | Reconstitute with 50µl of Reconstitution Solution (water containing 0.1% sodium azide) to a final concentration of 2mg/ml. Further dilutions should be made using 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide. |
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Use/Stability: | Reconstituted solution can be stored at 4°C for 6 months and at -20°C for 1 year. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Undercarboxylated, But Not Carboxylated, Osteocalcin Suppresses TNF-α-Induced Infammatory Signaling Pathway in Myoblasts: D. Park, et al.; J. Endocr. Soc.
6, bvac084 (2022),
Abstract;
Circadian disruption by shifting the light-dark cycle negatively affects bone health in mice: M. Schilperoort, et al.; FASEB J.
34, 1052 (2019),
Abstract;
Full Text
Insulin-Like Growth Factor I Does Not Drive New Bone Formation in Experimental Arthritis: M.N. van Tok, et al.; PLoS One
11, e0163632 (2016),
Application(s): Immunohistochemistry, ankle and tow section osteoblasts,
Abstract;
Full Text
Macrophages Promote Osteoblastic Differentiation In-Vivo: Implications in Fracture Repair and Bone Homeostasis: L. Vi, et al.; J. Bone Miner. Res.
30, 1090 (2015),
Application(s):IHC on FFPE sections of mouse tibia,
Abstract;