| Host: | Rabbit |
| |
| Immunogen: | Synthetic peptide corresponding to aa 58-70 (V58EDINKRREPIPS70) of human Munc-18. |
| |
| UniProt ID: | P61764 (Munc-18-1), Q15833 (Munc-18-2) |
| |
| Species reactivity: | Mouse, Rat
|
| |
| Applications: | IHC (FS), IP, WB
|
| |
| Recommended Dilutions/Conditions: | Western Blot (1µg/ml) |
| |
| Application Notes: | Detects a band of ~67kDa by Western blot. |
| |
| Purity Detail: | Epitope-affinity purified IgG. |
| |
| Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide. |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | Munc-18 is a cytosolic brain protein of ~67kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc-18, SNAP-25, synaptobrevin, and syntaxin. Munc-18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in unc-18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc-18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc-18 isoforms have been identified. Munc-18-1 has been shown to be primarily expressed in brain, while Munc-18-2 is expressed in most tissues. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
