Replaces Prod. #: ALX-210-239/1
Product Details
| Alternative Name: | EEA1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 1391-1410 (T1391PSSKKPVR
VCDACFNDLQG1410) of human EEA1 (early endosomal antigen 1). |
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| UniProt ID: | Q15075 |
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| Species reactivity: | Mouse
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| Applications: | WB
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| Application Notes: | Detects a band of ~160kDa by Western blot. |
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| Purity Detail: | Epitope-affinity purified IgG. |
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| Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | EEA1 is a 162kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains reminiscent of those found in nucleic acid binding proteins are located in the amino and carboxyl-terminal domains of EEA1. The COOH-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown to be dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Endocytic trafficking of GAS6-AXL complexes is associated with sustained AKT activation: A. Poświata, et al.; Cell. Mol. Life Sci.
79, 316 (2022),
Abstract;
Vps11 and Vps18 of Vps-C membrane traffic complexes are E3 ubiquitin ligases and fine-tune signalling: G. Segala, et al.; Nat. Commun.
10, 1833 (2019),
Abstract;
Full Text
Severe hypochromic microcytic anemia caused by a congenital defect of the iron transport pathway in erythroid cells: M. Priwitzerova, et al.; Blood
103, 3991 (2004),
Abstract;
