Product Details
Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 780-793 (R780GERTAFIKDQSAL793) of C-terminal human furin. |
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UniProt ID: | P09958 |
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Species reactivity: | Mouse Dog
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Specificity: | Recognizes mouse and dog furin convertase and overexpressed human furin convertase. Does not detect endogenous furin from BSC-40, HeLa, J774A-1 BPAEC or CHO cells. |
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Crossreactivity: | Does not cross-react with rat furin convertase. |
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Applications: | ICC, IF, IP, WB
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Recommended Dilutions/Conditions: | Immunoprecipitation (1:100) Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunocytochemical staining of endogenous furin convertase in 3T3 cells with Prod. No. ALX-210-134 yields a pattern consistent with paranuclear staining.
Detects a band of ~100kDa by Western blot. |
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Purity Detail: | Epitope-affinity purified IgG. |
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Formulation: | Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure: Immunolocalization of endogenous furin in mouse 3T3 cells using PAb to Furin Convertase (Prod. No.
ALX-210-134)."
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Product Literature References
Shedding of membrane type matrix metalloproteinase 5 by a furin-type convertase: a potential mechanism for down-regulation: X. Wang & D. Pei; J. Biol. Chem.
276, 35953 (2001),
Abstract;
Full Text
Cytoskeletal protein ABP-280 directs the intracellular trafficking of furin and modulates proprotein processing in the endocytic pathway: G. Liu, et al.; J. Cell Biol.
139, 1719 (1997),
Abstract;
Full Text