Product Details
Alternative Name: | Phosphodiesterase 5A, cGMP-specific 3',5'-cyclic phosphodiesterase, CGB-PDE |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 857-875 (CA857EQQEKMLINGESGQAKRN875) of human PDE5A (phosphodiesterase 5A). |
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UniProt ID: | O76074 |
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Species reactivity: | Mouse Bovine
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Applications: | WB
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Recommended Dilutions/Conditions: | Western Blot (1:10,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Epitope-affinity purified. |
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Formulation: | Liquid. In PBS containing 30% glycerol and 0.02% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Figure 1: Western blot using PAb to PDE5A (Prod. No.
ALX-210-099) at 1:1’000 dilution.Lane 1) 100µg of PDE5A-deficient SKN-BE2 neuroblastoma lysate. Lane 2) 100µg lysate mixed with 5ng of purified recombinant bovine PDE5A (Prod. No.
ALX-201-257). Lane 3) 3ng of partially purified recombinant bovine PDE5A. Signal was developed for 5 sec. using ECL-Plus reagent (Amersham)."
Figure 2: Western blot using PAb to PDE5A (Prod. No.
ALX-210-099) at 1:1’000 dilution.Lane 1) 200µg of 15’000 x g supernatant of lysate from mouse brain. Lane 2) Mouse heart. Lane 3) Mouse lung. Lane 4) Mouse liver. Lane 5) 5ng of purified bovine PDE5."
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Product Literature References
The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus: C. Mátyás, et al.; Cardiovasc. Diabetol.
14, 145 (2015),
Application(s): Immunoblot analysis,
Abstract;
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