Product Details
| Alternative Name: | Bcl-2 associated athanogene-1, BAG family molecular chaperone regulator 1, RAP46 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 204-219 (C204QETERLQSTNLALAE219) of mouse BAG-1 (Bcl-2 associated athanogene-1). |
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| UniProt ID: | Q60739 |
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| Species reactivity: | Mouse
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| Applications: | IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections, 1:2'500)
Immunoprecipitation (transfected cells)
Western Blot (1:500-1:1,000).
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions should be determined individually for each application. |
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| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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| Use/Stability: | Stable for at least 1 year after receipt when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ.
9, 405 (2002),
Abstract;
Role of Bag-1 in the survival and proliferation of the cytokine-dependent lymphocyte lines, Ba/F3 and Nb2: C.V. Clevenger, et al.; Mol. Endocrinol.
11, 608 (1997),
Abstract;
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Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1: H.G. Wang, et al.; PNAS
93, 7063 (1996),
Abstract;
Full Text
Interleukin-2 (IL-2) upregulates BAG-1 gene expression through serine-rich region within IL-2 receptor beta c chain: M. Adachi, et al.; Blood
88, 4118 (1996),
Abstract;
Full Text
Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity: S. Takayama, et al.; Cell
80, 279 (1995),
Abstract;
General Literature References
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol.
37, 251 (2005),
Abstract;
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones
8, 225 (2003),
Abstract;
Full Text
