Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Annexin V (human), (recombinant) (APC conjugate)

 
ALX-209-252-T020 20 tests 142.00 USD
 
ALX-209-252-T100 100 tests 415.00 USD
Do you need bulk/larger quantities?
 
APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).

Product Details

MW:~35.8kDa
 
Source:Produced in E. coli.
 
UniProt ID:P08758
 
Quantity:20 tests/100µl; 100 tests/500µl.
 
Formulation:Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
 
Purity:≥89% (SDS-PAGE, HPLC)
 
Specificity:Binds to phosphatidylserine (PS).
 
Applications:Flow Cytometry
 
Application Notes:Detection of apoptotic cells by flow cytometry.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Handling:Protect from light. Do not freeze.
 
Protocol:Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm pore filter);
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-APC
- Mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- FACS analysis
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Small molecules baicalein and cinnamaldehyde are potentiators of measles virus-induced breast cancer oncolysis: Y. T. Kuo, et al.; Phytomedicine 89, 153611 (2021), Abstract;
In vitro and in vivo efficacy of non-psychoactive cannabidiol in neuroblastoma: T. Fisher, et al.; Curr. Oncol. 23, S15 (2016), Application(s): Annexin V assay, Abstract; Full Text

Related Products

Annexin V (human), (recombinant) (FITC conjugate) 

Produced in E. coli., ≥98% (SDS-PAGE, HPLC), Flow Cytometry | Print as PDF
 
ALX-209-250-T020 20 tests 142.00 USD
 
ALX-209-250-T100 100 tests 522.00 USD
 
ALX-209-250-T300 300 tests 788.00 USD
Do you need bulk/larger quantities?
 

Annexin V (human), (recombinant) (R-PE conjugate) 

Produced in E. coli., ≥89% (SDS-PAGE, HPLC), Flow Cytometry | Print as PDF
 
ALX-209-251-T020 20 tests 142.00 USD
 
ALX-209-251-T100 100 tests 415.00 USD
Do you need bulk/larger quantities?
 

Annexin V (human), (recombinant) (FITC conjugate) 

Produced in E.coli. , ≥90%, Flow Cytometry | Print as PDF
 
ALX-209-256-T100 100 tests 238.00 USD
 
ALX-209-256-3100 3x100 tests 546.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
SDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Annexin
By biological activity:
Annexin Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

All new literature pieces