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Annexin V (human), (recombinant) (APC conjugate)



ALX-209-252-T020	20 tests	156.00 USD

ALX-209-252-T100	100 tests	457.00 USD
Do you need bulk/larger quantities?

APC-labelled recombinant human annexin V is suitable for dual staining experiments to detect phosphatidylserine whilst cell-surface protein can be detected with a suitable MAb (FITC-labelled or labelled secondary antibody). The fluorochrome APC (allophycocyanin) shows far red fluorescence (Ex(max): 650nm; Em(max): 660nm).

Product Details

MW:	~35.8kDa

Source:	Produced in E. coli.

UniProt ID:	P08758

Quantity:	20 tests/100µl; 100 tests/500µl.

Formulation:	Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.

Purity:	≥89% (SDS-PAGE, HPLC)

Specificity:	Binds to phosphatidylserine (PS).

Applications:	Flow Cytometry

Application Notes:	Detection of apoptotic cells by flow cytometry.

Shipping:	Blue Ice Not Frozen

Long Term Storage:	+4°C

Handling:	Protect from light. Do not freeze.

Protocol:	Flow Cytometry Protocol: - Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through 0.2µm pore filter); - Brief centrifugation before use is recommended - Wash cells in PBS by gentle shaking or pipetting up and down - Resuspend cells in binding buffer; adjust cell density to 2-5x10⁵/ml - Take 195µl cell suspension and add 5µl Annexin V-APC - Mix and incubate 10 min. in the dark at room temperature - Wash cells 1x with PBS and resuspend in 190µl binding buffer - FACS analysis

Regulatory Status:	RUO - Research Use Only

Product Literature References

Small molecules baicalein and cinnamaldehyde are potentiators of measles virus-induced breast cancer oncolysis: Y. T. Kuo, et al.; Phytomedicine 89, 153611 (2021), Abstract;

In vitro and in vivo efficacy of non-psychoactive cannabidiol in neuroblastoma: T. Fisher, et al.; Curr. Oncol. 23, S15 (2016), Application(s): Annexin V assay, Abstract; Full Text

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