FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
Product Details
| MW: | ~35.8kDa. |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P08758 |
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| Quantity: | 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl. |
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| Formulation: | Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide. |
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| Purity: | ≥98% (SDS-PAGE, HPLC) |
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| Specificity: | Binds to phosphatidylserine (PS). |
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| Biological Activity: | Exhibits anti-phospholipase activity. |
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| Applications: | Flow Cytometry
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| Application Notes: | Detection of apoptotic cells by flow cytometry. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Handling: | After opening, prepare aliquots and store at -20°C. |
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| Protocol: | Flow Cytometry Protocol:
- Prepare binding buffer (10mM Hepes/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm pore filter).
- Brief centrifugation of annexin V-FITC before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down.
- Resuspend cells in binding buffer; adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl annexin V-FITC
- Mix and incubate 10 min. in the dark at room temperature
- Wash cells 1x with PBS and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1µg/ml)
- FACS analysis |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The microvesicle/CD36 Complex Triggers a Prothrombotic Phenotype in Patients With Non-Valvular Atrial Fibrillation: H. Wang, et al.; J. Cell. Mol. Med.
24, 7331 (2020),
Abstract;
Full Text
Novel Compounds Targeting the Mitochondrial Protein VDAC1 Inhibit Apoptosis and Protect Against Mitochondria Dysfunction: D. Ben-Hail, et al.; J. Biol. Chem.
291, 24986 (2016),
Application(s): Flow cytometry using propidium iodide (PI) and annexin V-FITC staining,
Abstract;
Full Text
VDAC1-interacting anion transport inhibitors inhibit VDAC1 oligomerization and apoptosis: D. Ben-Hail, et al.; Biochim. Biophys. Acta
1863, 1612 (2016),
Application(s): Flow cytometry,
Abstract;
Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells: C. Amantini, et al.; Oncoscience
2, 395 (2015),
Application(s): FACS,
Abstract;
Full Text
Ca 2+-mediated regulation of VDAC1 expression levels is associated with cell death induction: S. Weisthal, et al.; Biochim. Biophys. Acta
1843, 2270 (2014),
Abstract;
Increased growth-inhibitory and cytotoxic activity of arsenic trioxide in Head and Neck carcinoma cells with functional p53 deficiency and resistance to EGFR blockade: M. Boyko-Fabian, et al.; PLoS One
9, e98867 (2014),
Abstract;
Full Text
4-Nonylphenol triggers apoptosis and affects 17-β-Estradiol receptors in calvarial osteoblasts: M.G. Sabbieti, et al.
; Toxicology
290, 334 (2013),
Abstract;
Cytoprotective effects of opioids on irradiated oral epithelial cells: N. Charbaji, et al.; Wound Repair Regen.
21, 883 (2013),
Abstract;
Dealcoholated red wine induces autophagic and apoptotic cell death in an osteosarcoma cell line: I. Tedesco, et al.; Food Chem. Toxicol.
60, 377 (2013),
Abstract;
Patients affected by metabolic syndrome show decreased levels of circulating platelet derived growth factor (PDGF)-BB: V. Tisato, et al.; Clin. Nutr.
32, 259 (2013),
Abstract;
The role of calcium in VDAC1 oligomerization and mitochondria-mediated apoptosis : N. Keinan, et al.; Biochim. Biophys. Acta
1833, 1745 (2013),
Abstract;
VDAC1-based peptides: novel pro-apoptotic agents and potential therapeutics for B-cell chronic lymphocytic leukemia: T. Prezma, et al.; Cell Death Dis.
4, e809 (2013),
Abstract;
Full Text
Inactivation of Bcl-2 through IκB kinase (IKK)-dependent phosphorylation mediates apoptosis upon exposure to 4-hydroxynonenal (HNE): C. Bodur, et al.; J. Cell. Physiol.
227, 3556 (2012),
Abstract;
Zoledronic acid suppresses mineralization through direct cytotoxicity and osteoblast differentiation inhibition: S. Patntirapong, et al.; J. Oral Pathol. Med.
41, 713 (2012),
Abstract;
NF-κB targeting by way of IKK inhibition sensitizes lung cancer cells to adenovirus delivery of TRAIL: C. Aydin, et al.; BMC Cancer
10, 584 (2010),
Abstract;
Full Text
DcR2 (TRAIL-R4) siRNA and adenovirus delivery of TRAIL (Ad5hTRAIL) break down in vitro tumorigenic potential of prostate carcinoma cells: A.D. Sanlioglu, et al.; Cancer Gene Ther.
14, 976 (2007),
Abstract;
CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol.
156, 4631 (1996),
Abstract;
Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry
24, 123 (1996),
Abstract;
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth.
184, 39 (1995),
Abstract;
Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med.
182, 1545 (1995),
Abstract;
Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood
85, 532 (1995),
Abstract;
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood
84, 1415 (1994),
Abstract;
Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol.
148, 2207 (1992),
Abstract;
Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem.
265, 4923 (1990),
Abstract;
Full Text
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