Product Details
| MW: | ~35.8kDa. |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P08758 |
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| Quantity: | 20 tests/100µl; 100 tests/500µl; 300 tests/1'500µl. |
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| Formulation: | Liquid. In 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide. |
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| Purity: | ≥98% (SDS-PAGE, HPLC) |
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| Specificity: | Binds to phosphatidylserine (PS). |
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| Biological Activity: | Exhibits anti-phospolipase activity. |
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| Applications: | Flow Cytometry
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| Application Notes: | Detection of apoptotic cells by flow cytometry. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | +4°C |
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| Handling: | Do not freeze. |
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| Protocol: | Flow Cytometry Protocol:
- Brief centrifugation before use is recommended
- Wash cells in PBS by gentle shaking or pipetting up and down
- Resuspend cells in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl2; filtered through 0.2µm pore filter); adjust cell density to 2-5x105/ml
- Take 195µl cell suspension and add 5µl Annexin V-Biotin
- Mix and incubate 15 minutes at room temperature
- Wash cells 2x in binding buffer
- Resuspend in binding buffer
- Add Streptavidin-FITC
- Mix and incubate for 30 minutes in the dark at room temperature
- Wash cells 1x in binding buffer and resuspend in 190µl binding buffer
- Add 10µl of 20µg/ml propidium iodide stock solution (end concentration 1mg/ml)
- FACS analysis |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Reducing VDAC1 expression induces a non-apoptotic role for pro-apoptotic proteins in cancer cell differentiation: T. Arif, et al.; Biochim. Biophys. Acta
1857, 1228 (2016),
Application(s): Apoptosis analysis,
Abstract;
CD95 engagement releases calcium from intracellular stores of long term activated, apoptosis-prone gammadelta T cells: P. Rovere, et al.; J. Immunol.
156, 4631 (1996),
Abstract;
Quantification of apoptotic cells with fluorescein isothiocyanate- labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin: A.W.M. Boersma, et al.; Cytometry
24, 123 (1996),
Abstract;
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V: I. Vermes, et al.; J. Immunol. Meth.
184, 39 (1995),
Abstract;
Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl: S. Martin, et al.; J. Exp. Med.
182, 1545 (1995),
Abstract;
Human neutrophils lose their surface Fc gamma RIII and acquire Annexin V binding sites during apoptosis in vitro: C.H. Homburg, et al.; Blood
85, 532 (1995),
Abstract;
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis: G. Koopman, et al.; Blood
84, 1415 (1994),
Abstract;
Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages: V.A. Fadok, et al.; J. Immunol.
148, 2207 (1992),
Abstract;
Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers: H.A. Andree, et al.; J. Biol. Chem.
265, 4923 (1990),
Abstract;
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