Control compound for rmsMEGACD40L® (Prod. No. ALX-522-120).
Product Details
Alternative Name: | Tail-Adp, Tail Adiponectin, Adipocyte complement related protein of 30kDa headless |
|
MW: | ~25kDa |
|
Source: | Produced in HEK 293 cells. The collagen domain of mouse ACRP30 (aa 18-111) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag. |
|
UniProt ID: | Q60994 |
|
Formulation: | Lyophilized. Contains PBS. |
|
Purity: | ≥90% (SDS-PAGE) |
|
Purity Detail: | Purified by multi-step chromatography. |
|
Endotoxin Content: | <0.1EU/µg protein (LAL-test). |
|
Application Notes: | Negative control for fusion proteins containing mouse ACRPheadless. |
|
Reconstitution: | Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. |
|
Shipping: | Shipped on Blue Ice |
|
Long Term Storage: | -20°C |
|
Use/Stability: | Stable for at least 6 months after receipt when stored as recommended. |
|
Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
|
Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
|
Regulatory Status: | RUO - Research Use Only |
|
Figure: ACRP30
headless (mouse) does not bind to rhTNF-R1 and rhOX40:Fc in an ELISA assay.
Method: A 96 well ELISA plate was coated O/N at RT with 100 µl of 1 µg/ml rhTNF-R1:Fc (Prod. No.
ALX-522-013) and 1 µg/ml rhOX40:Fc (Prod. No.
ALX-522-022) in PBS. 100 µl of rmsACRP30
headless at 8 µg/ml were mixed with 100 µl 0.5% FCS in PBS in the first well and a series of 1:1 dilutions was prepared for the following wells. Binding of rmsACRP30
headless to the receptor:Fc was detected after 1 hr incubation at 37°C using a goat anti-human HRP conjugated antibody (Jackson lab; 1:1
Please mouse over
Related Products