Control compound. When used at 1µg/g body weight in mouse demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in an in vivo assay.
Product Specification
| Alternative Name: | Adipocyte complement related protein of 30kDa headless, Tail-Adp, Tail Adiponectin |
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| MW: | ~27kDa |
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| Source: | Produced in HEK 293 cells. The non-homologous (aa 16-45) and collagen domain of human ACRP30 (aa 45-108) are fused at the N-terminus to a FLAG®-tag. |
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| UniProt ID: | Q15848 |
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| Formulation: | Lyophilized. Contains PBS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Endotoxin Content: | <0.1EU/µg protein (LAL-test). |
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| Application Notes: | Negative control. When used at 1µg/g body weight in mouse demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in an in vivo assay. |
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| Reconstitution: | Reconstitute with 100µl sterile water. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 6 months after receipt when stored as recommended. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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Figure 1: Bioactivity of rmsAdiponectin.
Method: 2 groups of 4 mice each were injected with PBS (vehicle), rmAdiponectin (Prod. No.
ALX-522-059) and rhACRP30
headless (tail adiponectin) (Prod. No.
ALX-203-002) at 1µg/g body weight as a tail vein injection. Subsequently, serum glucose levels were monitored over the time indicated by Trinder assay. Plotted is the average of 4 mice. rmAdiponectin represses hepatic glucose production, so serum glucose levels decrease until counter-regulatory mechanisms (i.e. glucagon) become effective. rhACRP30
headless demonstrated no significant bioactivity in terms of reduction of serum glucose compared to wildtype in this
in vivo assay."
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Product Literature References
Adiponectin acts in the brain to decrease body weight: Y. Qi, et al.; Nat. Med.
10, 524 (2004),
Abstract;
Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin: Implications for metabolic regulation and bioactivity: U.B. Pajvani, et al.; J. Biol. Chem.
278, 9073 (2003),
Abstract;
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