Potent immunoactivator commonly used as a carrier in the conjugation of peptides for antibody production. This preparation of chromatographically purified hemocyanin has been treated to reduce endotoxins for immunization protocols. Upon reconstitution with PBS a highly soluble solution is obtained for efficient and direct use for peptide coupling.
Product Specification
| Alternative Name: | Keyhole limpet hemocyanin |
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| Source: | Isolated from giant keyhole limpet (Megathura crenulata). |
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| UniProt ID: | P81732 |
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| Formulation: | Lyophilized from BES buffer containing MgSO4. |
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| Purity: | ≥90% (size exclusion chromatography). Stable solid containng ≥60% protein. Protein is 90% undissociated hemocyanin. |
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| Endotoxin Content: | ≤50EU/mg protein. |
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| Reconstitution: | Soluble in PBS or other physiological buffer at 5-10 mg/mL. Note that material is supplied as gross weight. |
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| Shipping: | Ambient |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
Keyhole limpet hemocyanin (KLH): a biomedical review: J.R. Harris & J. Markl; Micron
30, 597 (1999), (Review),
Abstract;
