Replaces Prod. #: BML-SE179
Product Details
| Alternative Name: | Poly(ADP-ribose) glycohydrolase |
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| Source: | Isolated from calf thymus. |
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| UniProt ID: | O02776 |
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| Formulation: | Liquid. In 50mM K-PO4 buffer, pH 7.5, containing 50mM potassium chloride, 0.1% Triton-x 100, 10% glycerol and 1mM DTT, 10mM β-Mercaptoethanol. |
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| Purity Detail: | Purified by substrate chromatography. The enzyme is composed of two major bands at 74kDa and 59kDa. |
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| Specific Activity: | ~20’000U/mg protein. 1U is defined as the amount of enzyme that hydrolyzes 1nmol of poly(ADP-ribose) per min. at 37°C. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | For further dilution use the following enzyme dilution buffer: 50mM KPO4, pH 7.5, 50mM KCl, 10 mM β-mercaptoethanol, 10% glycerol, 1mM DTT, 0.1% Triton. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Poly(ADP-ribosylation) is present in murine sciatic nerve fibers and is altered in a Charcot-Marie-Tooth-1E neurodegenerative model: L.I. Hughes, et al.; PeerJ
5, e3318 (2017),
Abstract;
Full Text
Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta
1844, 2222 (2014),
Abstract;
A family of macrodomain proteins reverses cellular mono-ADP-ribosylation: G. Jankevicius, et al.; Nat. Struct. Mol. Biol.
20, 508 (2013),
Abstract;
Full Text
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo: G.M. Shah et al.; Anal. Biochem.
227, 1 (1995),
Abstract;
Mode of action of poly(ADP-ribose) glycohydrolase: G. Brochu, et al.; Biochim. Biophys. Acta
1219, 342 (1994),
Abstract;
Purification of poly(ADP-ribose) glycohydrolase and detection of its isoforms by a zymogram following one- or two-dimensional electrophoresis: G. Brochu et al.; Anal. Biochem
218, 265 (1994),
Abstract;
Poly(ADP-ribosyl)ation of chromatin in an in vitro poly(ADP-ribose)-turnover system: H. Thomassin, et al.; Biochim. Biophys. Acta
1137, 171 (1992),
Abstract;
An affinity matrix for the purification of poly(ADP-ribose) glycohydrolase: H. Thomassin, et al.; Nucl. Acid Res.
18, 4691 (1990),
Abstract;
Rapid assay of poly(ADP-ribose) glycohydrolase: L. Menard & G.G. Poirier; Biochem. Cell Biol.
65, 668 (1987),
Abstract;
