Product Details
Alternative Name: | GTP pyrophosphate-lyase (cycling) |
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Source: | Isolated from bovine lung by an immunoaffinity method. |
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EC: | 4.6.1.2 |
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Concentration: | |
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Formulation: | Liquid. In ~20µl containing 50% glycerol, 88mM NaCl, 1mM DTT, 0.5mM EDTA, 25mM triethanolamine at pH 7.4. |
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Specific Activity: | >10'000nmol cGMP/mg/min at 37°C under NO-stimulated conditions. |
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Shipping: | Dry Ice |
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Long Term Storage: | -80°C |
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Use/Stability: | Product should be active for at least one year after shipping date when stored at -80°C under nitrogen in the presence of 50% glycerol. Dilutions of the enzyme should be peformed in the presence of 0.5mg/ml BSA. The dissolved product is stable for 8 months when stored in 50% glycerol at -80°C. |
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Handling: | Avoid freeze/thaw cycles. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A new small molecule inhibitor of soluble guanylate cyclase: F. Mota, et al.; Bioorg. Med. Chem.
23, 5303 (2015),
Abstract;
Full Text
Detection of nitric oxide production in cell cultures by luciferin-luciferase chemiluminescence: Y. Woldman, et al.; Biochem. Biophys. Res. Commun.
465, 232 (2015),
Application(s): Cell culture,
Abstract;
Direct chemiluminescence detection of nitric oxide in aqueous solutions using the natural nitric oxide target soluble guanylyl cyclase: Y.Y. Woldman, et al.; Free Radic. Biol. Med.
47, 1339 (2009),
Abstract;
Full Text
Purification of soluble guanylyl cyclase from bovine lung by a new immunoaffinity chromatographic method: P. Humbert, et al.; Eur. J. Biochem.
190, 273 (1990),
Abstract;