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FGF-basic 154 (human), (recombinant)

 
ALX-201-823-0010 10 µg 180.00 USD
 
ALX-201-823-0050 50 µg 273.00 USD
 
ALX-201-823-0100 100 µg 784.00 USD
Do you need bulk/larger quantities?
 
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Product Details

Alternative Name:FGF2, HBGF-2, Prostatropin
 
MW:~17.2kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 154 amino acids.
 
UniProt ID:P09038 + 7 aa of propeptide
 
Formulation:Lyophilized from 10mM sodium phosphate, 75mM NaCl, pH 7.5. Sterile filtered.
 
Purity:≥95% (Reducing and Non-reducing SDS-PAGE)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <1ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient Temperature
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Fibroblast growth factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 154, also known as FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems.
 
Regulatory Status:RUO - Research Use Only
 
FGF-basic 154 (human), (recombinant) image
Figure 1: Serial dilutions of Human FGF Basic 154, starting at 80 ng/mL, were added to NIH 3T3 cells. Cell proliferation was measured after 48 hours and the linear portion of the curve can be used to calculate the ED50.
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FGF-basic 154 (human), (recombinant) image

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