| Alternative Name: | Interleukin-3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor |
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| MW: | ~15.1kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 135 amino acids. |
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| UniProt ID: | P01586 |
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| Formulation: | Lyophilized. From sterile filtered solution containing 0.1% TFA. |
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| Purity: | ≥95% (reducing and non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent stimulation of TF-1 cells which is typically observed at concentations <0.1ng/ml. |
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| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Interleukin-3 (IL-3) is a cytokine produced by activated T cells and mast cells. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). IL-3 also has functions in the nervous system and appears to be important in several chronic inflammatory diseases. |
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| Regulatory Status: | RUO - Research Use Only |
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