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IL-3 (mouse), (recombinant)

ALX-201-821-0010 10 µg 273.00 USD
ALX-201-821-0100 100 µg 1,181.00 USD
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Product Details

Alternative Name:Interleukin-3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor
Source:Produced in E. coli. Non-glycosylated protein, containing 135 amino acids.
UniProt ID:P01586
Formulation:Lyophilized. From sterile filtered solution containing 0.1% TFA.
Purity:≥95% (reducing and non-reducing SDS-PAGE)
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
Appearance:White lyophilized (freeze-dried) powder.
Biological Activity:The activity is determined by the dose-dependent stimulation of TF-1 cells which is typically observed at concentations <0.1ng/ml.
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:Interleukin-3 (IL-3) is a cytokine produced by activated T cells and mast cells. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). IL-3 also has functions in the nervous system and appears to be important in several chronic inflammatory diseases.
Regulatory Status:RUO - Research Use Only
IL-3 (mouse), (recombinant) Bioactivity
Figure 1: Mouse IL-3 Bioactivity Data. Serial dilutions of mouse IL-3, starting at 0.350ng/ml, were added to Baf/3 cells. After 41 hours cell proliferation was measured and the linear portion of the curve was used to determine the ED50. The ED50 for this lot of mouse IL-3 was 0.3-0.47ng/ml. This value is comparable to the expected range of less than 0.5ng/ml.
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IL-3 (mouse), (recombinant) Bioactivity

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IL-3 (human), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 134 amino acids., ≥95% (Reducing and Non-reducing SDS-PAGE) | Print as PDF
ALX-201-820-0010 10 µg 314.00 USD
ALX-201-820-0100 100 µg 979.00 USD
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