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IL-6 (human), (recombinant)

ALX-201-818-0020 20 µg 221.00 USD
ALX-201-818-0100 100 µg 803.00 USD
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  • Carrier-free

Product Specification

Alternative Name:Interleukin-6, IFN-β2, B-cell differentiation factor, BSF-2, HSF, MGI-2
Source:Produced in E. coli. Non-glycosylated protein, containing 184 amino acids.
UniProt ID:P05231
Formulation:Lyophilized from 10mM acetic acid. Sterile filtered.
Purity:≥95% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
Appearance:White lyophilized (freeze-dried) powder.
Biological Activity:The activity is determined by the dose-dependant stimulation of mouse 7TD1 cells which is typically observed at concentrations <1ng/ml.
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 10 mM HCl at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Long Term Storage:-20°C
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells.
Regulatory Status:RUO - Research Use Only
IL-6  (human), (recombinant) SDS-PAGE
Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human IL-6 has a predicted MW of 20.9 kDa.
IL-6  (human), (recombinant) image
Figure 2: B9 cells were cultured with dilutions of Human IL-6. Cell proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
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IL-6  (human), (recombinant) SDS-PAGE IL-6  (human), (recombinant) image

Product Literature References

TLR5 activation by flagellin induces doxorubicin resistance via interleukin-6 (IL-6) expression in two multiple myeloma cells: H.Y. Cho, et al.; Cell. Immunol. 289, 27 (2014), Abstract;

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