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Growth and differentiation factor-15 (human), (recombinant)

ALX-201-803-0005 5 µg 184.00 USD
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Product Details

Alternative Name:GDF-15, MIC-1, Placental TGF-β, Prostate differentiation factor
Source:Produced in E. coli. Non-glycosylated, disulfide linked homodimer, containing two identical 113 amino acid chains.
UniProt ID:Q99988
Formulation:Lyophilized. Sterile filtered.
Purity:≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
Appearance:White lyophilized (freeze-dried) powder.
Biological Activity:The activity is determined by the inhibition of DU-145 cells which is typically observed at a concentration of 1-2µg/ml.
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM acetic acid (AcOH) at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:Growth and differentiation factor 15 (GDF-15) is a TGF-β family member, made by the placenta and heart tissues, that has a role in regulating inflammatory and apoptotic pathways. GDF-15 has become an immerging marker of early heart disease and has the potential as being used as a molecule for screening patients for early heart failure.
Regulatory Status:RUO - Research Use Only
Growth and differentiation factor-15 (human), (recombinant) SDS-PAGE
Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human GDF-15 is predicted to be a homodimer with a predicted MW of 24.5 kDa.
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Growth and differentiation factor-15 (human), (recombinant) SDS-PAGE

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