| Alternative Name: | FMS-related tyrosine kinase 3 ligand |
| |
| MW: | ~18.6kDa |
| |
| Source: | Produced in E. coli. Non-glycosylated protein, containing 163 amino acids. |
| |
| UniProt ID: | P49772 |
| |
| Formulation: | Lyophilized. Sterile filtered. |
| |
| Purity: | ≥98% (Reducing and Non-reducing SDS-PAGE) |
| |
| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
| |
| Appearance: | White lyophilized (freeze-dried) powder. |
| |
| Biological Activity: | The activity is determined by the dose-dependent proliferation of mouse Aml5 cells which is typically observed at a concentration of 5-8ng/ml. |
| |
| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
| |
| Shipping: | Ambient Temperature |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
| |
| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
| |
| Scientific Background: | FMS-related tyrosine kinase 3 ligand (Flt-3 ligand) is a growth factor important for the proliferation of hematopoietic cells. Flt-3 ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like tyrosine kinase-3 (Flt-3). Flt-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt-3 ligand can stimulate the proliferation of cells expressing mouse Flt-3 receptors. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Mouse Flt-3 ligand has a predicted MW of 18.6 kDa.
Figure 1: Mouse Flt-3 ligand Bioactivity Data. Serial dilutions of mouse Flt-3 ligand, starting at 500ng/ml, were added to Aml5. Cell proliferation was measured after 65 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 of the specific lot shown here is 2.2 - 3.3ng/ml. This value is comparable with the typical expected range of 5-8ng/ml.
