Product Details
| Alternative Name: | Delta-like protein 4, Delta4 |
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| MW: | ~54kDa |
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| Source: | Produced in HEK 293 cells. Recombinant human sDLL-4 contains 498 amino-acid residues. |
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| UniProt ID: | Q9NR61 |
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| GenBank ID: | 54567 |
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| Formulation: | Lyophilized. |
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| Purity: | ≥95% (SDS-PAGE and HPLC) |
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| Endotoxin Content: | < 0.1 ng per μg of sDLL-4. |
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| Biological Activity: | The sDLL-4, when immobilized at concentrations >1.5 μg/mL will inhibit myogenesis in C2C12 cells. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted sDLL-4 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Scientific Background: | Human sDLL4 comprises the extracellular signaling domain of DLL, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors. DLL4 functions to specifically activate the Notch-1 and Notch-4 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. Targeted deletion of the DLL4 gene in mice resulted in severe vascular defects and death before birth. Up-regulation of DLL4 expression has been implicated in the vascular development of certain tumors. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
MSCs promote the development and improve the function of neonatal porcine islet grafts: S. He, et al.; FASEB J.
32, 3242 (2018),
Application(s): Western blot, IF,
Abstract;
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General Literature References
Delta-like ligand 4 (Dll4) is induced by VEGF as a negative regulator of angiogenic sprouting: I.B. Lobov, et al.; PNAS
104, 3219 (2007),
Abstract;
Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis: M. Hellstrom, et al.; Nature
445, 776 (2007),
Abstract;
Dll4, a novel Notch ligand expressed in arterial endothelium: J.R. Shutter, et al.; Genes Dev.
14, 1313 (2000),
Abstract;
Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol.
154, 785 (1999),
Abstract;
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