Product Details
| Alternative Name: | Transmembrane protease serine 11E, TMPRSS11E |
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| MW: | ~45kDa; a-subunit: ~13kDa; b-subunit: ~32kDa |
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| Source: | Produced in Drosophila Schneider 2 (S2) cells. Recombinant human DESC1 (aa 44-423) is fused at the C-terminus to a V5-His-tag. Produced by plasmid transfection expression. |
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| EC: | 3.4.21.- |
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| UniProt ID: | Q9UL52 |
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| Concentration: | 100nM |
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| Formulation: | Liquid. In 100 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride and 20% glycerol. |
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| Biological Activity: | Trypsin-like serine protease. |
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| Specific Activity: | ~2.5U/µl. One unit is defined at the amount of enzyme that releases 10pmol of AMC per min. at 37°C in the presence of 50µM Boc-Gln-Ala-Arg-AMC. |
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| Shipping: | Shipped on Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: HEPES 50mM, pH 7.4, 150mM NaCl, 5mg/ml BSA. Do not store diluted solutions. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J.
276, 2213 (2009),
Abstract;
General Literature References
Human DESC1 serine protease confers tumorigenic properties to MDCK cells and it is upregulated in tumours of different origin: C.G. Viloria, et al.; Br. J. Cancer
97, 201 (2007),
Abstract;
