Product Details
| Alternative Name: | Transmembrane protease serine 1, TMPRSS1 |
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| MW: | ~45kDa; a-subunit: ~15kDa; b-subunit: ~30kDa |
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| Source: | Produced in Drosophila Schneider 2 (S2) cells. Recombinant human hepsin (aa 45-417) is fused at the C-terminus to a V5-His-tag. Produced by plasmid transfection expression. |
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| EC: | 3.4.21.106 |
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| UniProt ID: | P05981 |
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| Concentration: | 100nM |
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| Formulation: | Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride and 10% glycerol. |
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| Biological Activity: | Trypsin-like serine protease. |
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| Specific Activity: | ~2.5U/µl. One unit is defined at the amount of enzyme that releases 1nmol of AMC per min. at 37°C in the presence of 50µM Boc-Gln-Ala-Arg-AMC. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: 100mM TRIS, pH 9.0, containing 5mg/ml BSA. Do not store diluted solutions. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J.
276, 2213 (2009),
Abstract;
General Literature References
HEPSIN inhibits cell growth/invasion in prostate cancer cells: V. Srikantan, et al.; Cancer Res.
62, 6812 (2002),
Abstract;
