Product Details
| Alternative Name: | Proepithelin, PEPI, PC cell-derived growth factor |
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| MW: | ~90-95kDa |
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| Source: | Produced in mouse myeloma cell line NS0. Human progranulin (aa 18-593) is fused at the C-terminus to a 6-His tag. |
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| UniProt ID: | P28799 |
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| Formulation: | Lyophilized from a 0.2 μm filtered solution in PBS. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <0.01EU/µg protein (LAL-test). |
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| Activity: | Measured by its ability to enhance neurite outgrowth of E16E18 rat embryonic cortical neurons. rhProgranulin, immobilized at 1040 μg/ml on a nitrocellulose coated plate, is able to significantly enhance neurite outgrowth. |
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| Reconstitution: | Reconstitute at 200μg/ml in sterile PBS. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Working aliquots are stable for up to 3 months when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Scientific Background: | Progranulin (PGRN) has been shown to be a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signalling cascades that control cell cycle progression and cell motility. PGRN has also been linked to tumorigenesis. The function of PGRN in the central nervous system (CNS) is also of interest, because mutations in the PGRN gene have been identified in cases of frontotemporal degeneration (FTD). |
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| Regulatory Status: | RUO - Research Use Only |
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General Literature References
Progranulin: normal function and role in neurodegeneration: J.L. Eriksen & I.R. Mackenzie; J. Neurochem.
104, 287 (2008), (Review),
Abstract;
Progranulin in frontotemporal lobar degeneration and neuroinflammation: Z. Ahmed, et al.; J. Neuroinflammation
4, 7 (2007), (Review),
Abstract;
Full Text
Progranulin (granulin-epithelin precursor, PC-cell-derived growth factor, acrogranin) mediates tissue repair and tumorigenesis: Z. He & A. Bateman; J. Mol. Med.
81, 600 (2003),
Abstract;
Progranulin is a mediator of the wound response: Z. He, et al.; Nat. Med.
9, 225 (2003),
Abstract;
Granulins, a novel class of peptide from leukocytes: A. Bateman, et al.; BBRC
173, 1161 (1990),
Abstract;
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