| Alternative Name: | Indoleamine 2,3-dioxygenase, INDO |
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| Source: | Produced in E. coli. Mature human IDO (indoleamine 2,3-dioxygenase) (aa 1-403) is fused at the C-terminus to a His-tag. |
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| EC: | 1.13.11.52 |
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| UniProt ID: | P14902 |
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| Formulation: | Liquid. 0.2µm-filtered solution in 50mM TRIS, pH 7.5, containing 100mM NaCl. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | <1.0EU/µg protein (LAL test; Bio Whittaker). |
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| Specific Activity: | > 200 U/μg. One unit is defined as the conversion of 1 pmol/min L-tryptophan to N-formylkynurenine. |
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| Applications: | WB
Activity assay
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| Application Notes: | Western blot control. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Working aliquots are stable for up to 12 months when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | Indoleamine 2,3-dioxygenase (IDO) is an enzyme that catalyzes the degradation of L-tryptophan to N-formylkynurenine. This immunomodulatory enzyme has been shown to allow tumor cells to escape the immune system by depletion of L-Trp in the microenvironment of cell A wide range of human cancers overexpress human IDO (hIDO). |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis - Lane 1: MW Marker, Lane 2: 1 μg IDO. (Prod. No. ALX-201-501, IDO (human), (recombinant) (His-tag))
Western blot analysis – Lane 1: MW Marker, Lane 2: 100 ng IDO. Probed with pAb raised against full-length human recombinant IDO. (Prod. No. ALX-201-501, IDO (human), (recombinant) (His-tag)).
