| ALX-201-468-C010 | 10 µg | 521.00 USD |  |
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| Alternative Name: | Interleukin-33, NF-HEV, IL-1F11 |
| Source: | Produced in E. coli. The homeodomain of human IL-33 (aa 1-111) is fused at the C-terminus to a His-tag. |
| UniProt ID: | O95760 |
| Concentration: | 0.5mg/ml |
| Formulation: | Liquid. 0.2µm-filtered solution in 55mM TRIS-HCl, pH 8.2, containing 150mM sodium chloride. |
| Purity: | ≥95% (SDS-PAGE) |
| Purity Detail: | His-tag (Ni-NTA) and anion exchange chromatography purified. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Use/Stability: | Working aliquots are stable for up to 3 months when stored at -20°C. |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
| Scientific Background: | IL-33 gene was initially designated as NF-HEV which encodes a predicted 270-residue protein. The calculated 30 KDa human IL33 is converted by CASP1 to a mature 18-KD protein. IL-33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1(MAPK3)/ERK2(MAPK1), p38(MAPK14), and JNK. Nuclear localization, heterochromatinassociation, and targeting to mitotic chromosomes were all found to be mediated by an evolutionarily conserved homeodomain-like helix-turn-helix motif within the IL-33 N-terminal part. IL-33 was found to possess transcriptional repressor properties, associated with the homeodomain-like helix-turn-helix motif. |
| Regulatory Status: | RUO - Research Use Only |
