Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428).
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For initiation of differentiation, 3T3L-1 cells were cultured in growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX and 10μg/ml insulin (day 0). The Medium was changed every 2 days and staining with Oil Red O was typically performed on day 7. As negative control, human TNF-α (20ng/ml) was used. To immobilize Notch ligands in the culture plates, plates were incubated with a solution of DNER (Extracellular Domain) (human):Fc (human) (rec.) (5μg/ml) or hCD137(4-1BB)-Fc (5μg/ml). Visualized at three different spots.
