Product Specification
| Alternative Name: | Pre-B-cell colony enhancing factor 1, Nicotinamide phosphoribosyltransferase |
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| Source: | Produced in E. coli. Full length rat Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag. |
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| UniProt ID: | Q80Z29 |
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| Formulation: | Lyophilized from 0.2μm-filtered solution in 0.1M ammonium bicarbonate, pH 7.2. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <1EU/µg protein (LAL test). |
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| Biological Activity: | Shows adipogenic effect in differentiating 3T3-L1 cells. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Working aliquots are stable for up to 3 months when stored at -20°C. |
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| Handling: | For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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Figure 2: Adipogenic effect of Nampt (Visfatin/PBEF) (rat) (rec.) (His) (Prod. No. ALX-201-332) on differentiating 3T3-L1 cells.Method: 5µg/ml recombinant rat Nampt or human insulin was added to the differentiating 3T3-L1 cells that had been stimulated with 1µM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
Figure 1: SDS-PAGE of Nampt (Visfatin/PBEF) (rat) (rec.) (His) (Prod. No. ALX-201-332).
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For Research Use Only. Not for use in diagnostic procedures.
