Soluble, stable and more potent Ang-1 variant.
Product Details
| Alternative Name: | COMP:Ang-1 |
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| Source: | Produced in CHO cells. The fibrinogen-like domain (aa 284-498) of human Ang-1 (angiopoietin-1) is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag. |
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| UniProt ID: | Q15389 (human Angiopoietin-1), P35444 (rat COMP) |
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| Concentration: | ~0.2-0.4 mg/mL
(Please refer to Certificate of Analysis for lot-specific concentration.) |
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| Formulation: | Liquid. 0.2µm-filtered solution in PBS containing 0.01% CHAPS. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Endotoxin Content: | <0.1EU/µg protein (LAL test; BioWhittaker) |
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| Specificity: | Binds to human Tie-2. |
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| Biological Activity: | Induces phosphorylation of Tie-2 in primary cultured human and mouse vascular endothelial cells. More potent than native Ang-1. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Working aliquots are stable for up to 3 months when stored at -80°C. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Technical Info/Product Notes: | FLAG is a registered trademark of Sigma-Aldrich Co. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: COMP (rat):Angiopoietin-1 (human) (rec.) (Prod. No.
ALX-201-314) forms mainly pentameric structures.
Method: Glycerol spraying/low angle rotary metal-shadowed specimens were imaged by transmission electron microscopy."
Figure 2: COMP (rat):Angiopoietin-1 (human) (rec.) (Prod. No.
ALX-201-314) induces phosphorylation of Tie-2 in human umbilical vein endothelial cells.
Method: Serum-starved human umbilical vein endothelial cells were treated with 200ng/ml of BSA (Co) or 200ng/ml of COMP:Angiopoietin-1 for 15 min. The phosporylation of Tie-2 (phospho-Tie-2) was measured after immunoprecipitation."
Structural scheme of ALX-201-314: Produced in CHO cells. The fibrinogen-like domain (aa 284-498) of human Ang-1 (angiopoietin-1) is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.
SDS-PAGE analysis of ALX-201-314: Lane 1: MW Marker, Lane 2: 1µg of COMP (rat):Angiopoietin-1 (human), (recombinant) protein.
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Product Literature References
Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization: J.M. Ha, et al.; Exp. Mol. Med.
54, 1133 (2022),
Abstract;
Angiopoietin-1 deficiency increases tumor metastasis in mice: I.P. Michael, et al.; BMC Cancer
17, 539 (2017),
Abstract;
Full Text
Novel TIE-2 inhibitor BAY-826 displays in vivo efficacy in experimental syngeneic murine glioma models: H. Schneider, et al.; J. Neurochem.
140, 170 (2017),
Abstract;
Cartilage oligomeric matrix protein enhances the vascularization of acellular nerves: W.L. Cui, et al.; Neural Regen. Res.
11, 512 (2016),
Abstract;
Full Text
COMP-Ang1 enhances DNA synthesis and cell cycle progression in human periodontal ligament cells via Tie2-mediated phosphorylation of PI3K/Akt and MAPKs: S.S. Lim, et al.; Mol. Cell. Biochem.
416, 157 (2016),
Abstract;
COMP-Ang1 prevents periodontitic damages and enhances mandible bone growth in an experimental animal model: G. Bhattarai, et al.; Bone
92, 168 (2016),
Abstract;
Cartilage Oligomeric Matrix Protein Angiopoeitin-1 Provides Benefits During Nerve Regeneration In Vivo and In Vitro: L. Qiu, et al.; Ann. Biomed. Eng.
43, 2924 (2015),
Abstract;
Full Text
Effects of Angiopoietin-1 on Hemorrhagic Transformation and Cerebral Edema after Tissue Plasminogen Activator Treatment for Ischemic Stroke in Rats: K. Kawamura, et al.; Plos One
9, e98639 (2014),
Abstract;
Full Text
Angiopoietin-1 promotes LYVE-1-positive lymphatic vessel formation: T. Morisada, et al.; Blood
105, 4649 (2005),
Abstract;
Multiple angiopoietin recombinant proteins activate the Tie1 receptor tyrosine kinase and promote its interaction with Tie2: P. Saharinen, et al.; J. Cell Biol.
169, 239 (2005),
Abstract;
COMP-Ang1: a designed angiopoietin-1 variant with nonleaky angiogenic activity: C.H. Cho, et al.; PNAS
101, 5547 (2004),
Abstract;
Full Text
Designed angiopoietin-1 variant, COMP-Ang1, protects against radiation-induced endothelial cell apoptosis: C.H. Cho, et al.; PNAS
101, 5553 (2004),
Abstract;
Full Text
