Product Details
| Alternative Name: | nNOS, NOS I |
| |
| MW: | ~80kDa |
| |
| Source: | Produced in E. coli. |
| |
| EC: | 1.14.13.39 |
| |
| UniProt ID: | P29475 |
| |
| Concentration: | 10mg/ml |
| |
| Formulation: | Liquid. In 40mM bis-tris-propane, pH 7.8, containing 150mM sodium chloride and 0.1% BSA. |
| |
| Purity: | ≥90% (SDS-PAGE and heme spectral analysis) |
| |
| Specific Activity: | ≥35nmol/mg/mol protein. Amount assayed 150–500nM. Optimal conditions: 37°C, 40mM EPPS buffer, pH 7.6, 1mM NOHA, 0.5mM DTT, 30mM H2O2, 10units/ml superoxide dismutase, 0.5mg/ml BSA and variable concentrations of tetrahydrobioterin (0–1'000mM) in a final volume of 100ml. Reactions were initiated by 30mM H2O2 and stopped by 1'500 units of catalase. |
| |
| Shipping: | Dry Ice |
| |
| Long Term Storage: | -80°C |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored at -80°C. Keep stock vial on ice at all times! |
| |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Nitric-oxide synthase output state. Design and properties of nitric-oxide synthase oxygenase/FMN domain constructs: D.K. Ghosh, et al.; J. Biol. Chem.
281, 14173 (2006),
Abstract;
Full Text
Structures of nitric oxide synthase isoforms complexed with the inhibitor AR-R17477 suggest a rational basis for specificity and inhibitor design: R. Fedorov, et al.; PNAS
101, 5892 (2004),
Abstract;
Full Text
General Literature References
High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli: L.J. Roman, et al.; PNAS
92, 8428 (1995),
Abstract;
Full Text
Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer: H.M. Abu-Soud and D.J. Stuehr; PNAS
90, 10769 (1993),
Abstract;
Full Text
