Product Details
Alternative Name: | MT-SP1, Membrane-type serine protease 1, ST14, Suppression of tumorigenicity protein 14, TADG-15, Tumor-associated differentially expressed gene 15 protein, SNC19 |
|
MW: | ~25kDa |
|
Source: | Recombinant human matriptase (aa 596-855) fused at the N-terminus to a His-tag. |
|
UniProt ID: | Q9Y5Y6 |
|
Concentration: | 100nM |
|
Formulation: | Liquid. In 100mM TRIS, pH 8.0, containing 200mM sodium chloride, 1mM β-mercaptoethanol and 10% glycerol. |
|
Purity: | ≥98% (SDS-PAGE) |
|
Activity: | Trypsin-like serine protease. |
|
Specific Activity: | ~2.5U/µl. One unit is defined as the amount of enzyme that releases 10pmol of AMC per min. at 37°C in the presence of 50µM of Boc-Gln-Ala-Arg-AMC. |
|
Shipping: | Dry Ice |
|
Short Term Storage: | -20°C |
|
Long Term Storage: | -80°C |
|
Use/Stability: | Stable for at least 6 months when stored at -20°C. Do not store diluted solutions. |
|
Handling: | Avoid freeze/thaw cycles. |
|
Technical Info/Product Notes: | Recommended Assay Buffer is HEPES 50mM pH 7.4, 150 mM NaCl and 5mg/ml BSA |
|
Regulatory Status: | RUO - Research Use Only |
|
Please mouse over
Product Literature References
Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J.
276, 2213 (2009),
Abstract;
Inhibition of human matriptase by eglin c variants: A. Désilets, et al.; FEBS Lett.
580, 2227 (2006),
Abstract;
Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue: T. Takeuchi, et al.; PNAS
96, 11054 (1999),
Abstract;
Full Text