Replaces Prod. #: ALX-201-205/1
Product Details
| Alternative Name: | BMP-2 |
| |
| MW: | ~25.9kDa. |
| |
| Source: | Produced in E. coli and renatured from inclusion body material. |
| |
| UniProt ID: | P12643 |
| |
| Concentration: | 100-500μg/ml after reconstitution. |
| |
| Formulation: | Lyophilized. |
| |
| Purity: | ≥98% (SDS-PAGE, UV, MS) |
| |
| Endotoxin Content: | <0.02EU/mg protein. |
| |
| Biological Activity: | Induces alkaline phosphatase expression at an EC50 = 14nM in C2C12 cells. |
| |
| Reconstitution: | Reconstitute with 20-100μl 50mM sodium acetate solution, pH 4.3. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Use/Stability: | The reconstituted solution can be further diluted in sodium acetate solution and stored at +4°C for up to two weeks. Below 10μg/ml, 0.5% BSA or serum should be added. |
| |
| Handling: | After reconstitution, prepare aliquots and store at -80°C. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor : F. Hillger, et al.; J. Biol. Chem.
280, 14974 (2005),
Abstract;
Full Text
