Product Details
| Alternative Name: | GTP pyrophosphate-lyase (cycling), sGCα1/sGCβ1 |
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| Source: | Produced in Sf9 insect cells. Fused at the C-terminus to a His-tag. Produced in a baculovirus expression system. |
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| EC: | 4.6.1.2 |
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| UniProt ID: | Q02108 (α1 subunit), Q02153 (β1 subunit) |
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| GenBank ID: | AAB94794 (α1 subunit), AAH47620 (β1 subunit) |
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| Concentration: | ≥1.5mg/ml (total protein) |
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| Formulation: | Liquid. Enriched fraction in 50mM TEA, pH 7.4 containing 1mM EDTA/EGTA, 5mM GSH and 30% glycerol. |
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| Purity Detail: | Prepared by one column purification. |
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| Specific Activity: | - Basal: ≥0.001µmol/mg/min.
- 200µM DEA NONOate (Prod. No. ALX-430-034): ≥0.5µmol/mg/min.
- 1µM BAY41-2272 (Prod. No. ALX-420-030): ≥0.01µmol/mg/min. |
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| Application Notes: | Enzymatic studies and drug screening. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | We do not recommend to store for longer periods after opening and thawing of the product. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Soluble guanylyl cyclase mediates noncanonical nitric oxide signaling by nitrosothiol transfer under oxidative stress: C. Cui, et al.; Redox Biol.
55, 102425 (2022),
Abstract;
Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys.
388, 185 (2001),
Abstract;
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS
97, 10763 (2000),
Abstract;
Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med.
77, 14 (1999),
Abstract;
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J.
335 (Pt 1), 51 (1998),
Abstract;
Full Text
Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett.
132, 71 (1981),
Abstract;
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