Product Specification
| Alternative Name: | FLICE, Mch5, MACH |
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| Source: | Produced in E. coli. |
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| UniProt ID: | O89110 |
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| Concentration: | 1mg/ml |
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| Formulation: | Liquid. In 50mM HEPES, pH 7.4, containing 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 50% glycerol. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Specific Activity: | ≥100U/µg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate Z-IETD-AMC (AMC: 7-aminomethylcoumarin) (Prod. No. ALX-260-042) per hour at +30°C in a reaction solution containing 20µM Z-IETD-AMC, 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 10% glycerol. |
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| Shipping: | Shipped on Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stability at -20°C: 1-2 weeks. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Technical Info/Product Notes: | Note: Activity of this enzyme may essentially decrease after repeated freezing and thawing. For consistent results, it is recommended to use fresh aliquots. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Caspase-1 Engagement and TLR-Induced c-FLIP Expression Suppress ASC/Caspase-8-Dependent Apoptosis by Inflammasome Sensors NLRP1b and NLRC7: N. Van Opdenbosch, et al.; Cell Rep.
21, 3427 (2017),
Abstract;
Full Text
Identification and characterization of a novel mammalian caspase with proapoptotic activity: L. Eckhart, et al.; J. Biol. Chem.
280, 35077 (2005),
Abstract;
Full Text
General Literature References
Tumor Necrosis Factor-induced Toxic Liver Injury Results from JNK2-dependent Activation of Caspase-8 and the Mitochondrial Death Pathway: Y. Wang, et al.; J. Biol. Chem.
281, 15258 (2006),
Abstract;
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