Product Specification
| Alternative Name: | ICE-LAP-6, Mch6 |
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| Source: | Produced in E. coli. |
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| EC: | 3.4.22.- |
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| UniProt ID: | P55211 |
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| Concentration: | 0.2mg/ml |
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| Formulation: | Liquid. In 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least one week when stored at +4°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | When expressed in E. coli, caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-9 enzymatic activity can be measured by the release of AMC from the caspase substrate Ac-LEHD-AMC (Prod. No. ALX-260-080) as emission at 440nm and excitation at 380nm using a spectrofluorometer. |
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Product Literature References
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
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