Product Details
| Alternative Name: | Lipopolysaccharide binding protein |
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| Source: | Produced in CHO cells. |
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| UniProt ID: | P18428 |
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| Concentration: | 0.25mg/ml after reconstitution. |
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| Formulation: | Lyophilized. Contains PBS, pH 7.2. |
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| Purity: | ≥90-95% (SDS-PAGE) |
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| Endotoxin Content: | <0.00015EU/µg protein (LAL test; Charles River). |
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| Biological Activity: | Mediates binding of LPS to CD14 (up to 0.2µg/ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+ CHO transfectants (FACS)). |
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| Reconstitution: | Reconstitute with 40µl distilled water. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Will precipitate by rapid changes of pH or temperature. Best working temperature is ice bath. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | LBP (lipopolysaccharide binding protein) is a ~58kDa acute phase glycoprotein and binds to the lipid A portion of lipopolysaccharide (LPS) with high affinity and catalyzes the CD14 dependent cellular activation by LPS. |
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| Technical Info/Product Notes: | Note: His-tag at the C-terminal end of the LBP has no protease site and cannot be split off. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: SDS-PAGE of LBP (human) (rec.) (His) (Prod. No.
ALX-201-131)."
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Product Literature References
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol.
157, 4119 (1996),
Abstract;
