Product Details
| Alternative Name: | DPPIV, Dipeptidyl peptidase IV, DPP4 |
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| Source: | Produced in Sf9 insect cells. Produced in a baculovirus expression system. |
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| EC: | 3.4.14.5 |
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| UniProt ID: | P27487 |
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| Quantity: | 5mU |
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| Concentration: | 50µg/ml (Pierce - BCA) |
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| Formulation: | Liquid. In 20mM TRIS-HCl, pH 8.0, containing 5mM CaCl2, 1µM ZnCl2 and 0.05% sodium azide. |
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| Purity: | ≥95% (SDS-PAGE, Western blot). No other protease contaminants detectable. |
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| Specific Activity: | ~10U/mg protein. One unit is defined as the amount of enzyme that produces 1µmol p-nitroaniline or 7-amino-4-methylcoumarin per min. at 37°C in 100mM TRIS-HCl, pH 8.5, using H-Gly-Pro-pNA or H-Gly-Pro-AMC as a substrate respectively (enzyme dilution >1:500; incubation time 10-30 min.). |
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| Shipping: | Shipped on Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for up to 3 months when stored at -20°C. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Inhibitors: Very sensitive to diisopropyl fluorophosphate (DFP), less sensitive to other serine enzyme inhibitors such as phenylmethylsulfonyl fluoride (PMSF) (Prod. No. ALX-270-184) and diethyl 4-nitrophenylphosphate (E600). Most of the usual sulfhydryl reagents have little or no effect, nor do chelating agents. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
OL3, a novel low-absorbed TGR5 agonist with reduced side effects, lowered blood glucose via dual actions on TGR5 activation and DPP-4 inhibition: S.Y. Ma, et al. ; Acta Pharmacol. Sin.
37, 1359 (2016),
Application(s): In vitro DPP-4 activity assay,
Abstract;
Full Text
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