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United States 

CD26 (human), (recombinant)

ALX-201-128-1 1 Vial 469.00 USD
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Product Details

Alternative Name:DPPIV, Dipeptidyl peptidase IV, DPP4
Source:Produced in Sf9 insect cells. Produced in a baculovirus expression system.
UniProt ID:P27487
Concentration:50µg/ml (Pierce - BCA)
Formulation:Liquid. In 20mM TRIS-HCl, pH 8.0, containing 5mM CaCl2, 1µM ZnCl2 and 0.05% sodium azide.
Purity:≥95% (SDS-PAGE, Western blot). No other protease contaminants detectable.
Specific Activity:~10U/mg protein. One unit is defined as the amount of enzyme that produces 1µmol p-nitroaniline or 7-amino-4-methylcoumarin per min. at 37°C in 100mM TRIS-HCl, pH 8.5, using H-Gly-Pro-pNA or H-Gly-Pro-AMC as a substrate respectively (enzyme dilution >1:500; incubation time 10-30 min.).
Shipping:Shipped on Dry Ice
Short Term Storage:-20°C
Long Term Storage:-80°C
Use/Stability:Stable for up to 3 months when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Technical Info/Product Notes:Inhibitors: Very sensitive to diisopropyl fluorophosphate (DFP), less sensitive to other serine enzyme inhibitors such as phenylmethylsulfonyl fluoride (PMSF) (Prod. No. ALX-270-184) and diethyl 4-nitrophenylphosphate (E600). Most of the usual sulfhydryl reagents have little or no effect, nor do chelating agents.
Regulatory Status:RUO - Research Use Only

Product Literature References

OL3, a novel low-absorbed TGR5 agonist with reduced side effects, lowered blood glucose via dual actions on TGR5 activation and DPP-4 inhibition: S.Y. Ma, et al. ; Acta Pharmacol. Sin. 37, 1359 (2016), Application(s): In vitro DPP-4 activity assay, Abstract; Full Text

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Phenylmethylsulfonyl fluoride 

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ALX-270-184-G005 5 g 73.00 USD
ALX-270-184-G025 25 g 202.00 USD
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