Product Specification
| Alternative Name: | ProTα |
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| Source: | Produced in E. coli. Corresponding to the caspase-3 cleavage product of prothymosin α (ProTα) isolated from apoptotic human cells (no affinity-tags, no added additional (linker) sequences). Purified from overproducing bacterial strains by a phenol extraction procedure, DEAE chromatography, and ethanol precipitation. |
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| UniProt ID: | P06454 |
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| Concentration: | 1.5mg/ml |
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| Formulation: | Liquid. 50µg dissolved in sterile water. |
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| Purity: | ≥90% |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | The exact length of the human protein is 109 aa corresponding to the NCBl data base entries AAH34921 and TNHUA. The N-terminal M is absent in the mature protein (otherwise, it would be 110). ProTα is acetylated at the N-terminal S. MALDI-MS data for the recombinant ProTα isolated from bacteria show that the protein is partially acetylated. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Apoptosis-related fragmentation, translocation, and properties of human prothymosin alpha: A.G. Evstafieva, et al.; Exp. Cell Res.
284, 209 (2003),
Abstract;
Cytochrome c is transformed from anti- to pro-oxidant when interacting with truncated oncoprotein prothymosin alpha: O.V. Markova, et al.; Biochim. Biophys. Acta
1557, 109 (2003),
Abstract;
Sensing prothymosin alpha origin, mutations and conformation with monoclonal antibodies: E.A. Sukhacheva, et al.; J. Immunol. Methods
266, 185 (2002),
Abstract;
Overproduction in Escherichia coli, purification and properties of human prothymosin alpha: A.G. Evstafieva, et al.; Eur. J. Biochem.
231, 639 (1995),
Abstract;
