Product Details
| Alternative Name: | Apo-2L, TNFSF 10, CD253 |
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| Source: | Produced in E. coli. The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. |
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| UniProt ID: | P50591 |
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| Formulation: | Liquid. In 20mM HEPES, pH 7.4, containing 150mM sodium chloride, 0.005% Tween 20, 1% sucrose, 1mM DTT. |
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| Purity: | ≥98% (SDS-PAGE) |
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| Purity Detail: | Affinity purified. |
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| Endotoxin Content: | <10EU/µg protein (LAL test; Bio Whittaker). |
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| Species reactivity: | Human, Mouse
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| Specificity: | Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG). |
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| Biological Activity: | Induces apoptosis in a concentration range of 10-100ng/ml.
Note: This protein does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SUPERKILLERTRAIL® (Prod. No. ALX-201-115). |
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| Specific Activity: | ED50: 20ng/ml (BJAB cells) |
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| Reconstitution: | For a special storage and dilution buffer see KillerTRAIL™ Storage and Dilution Buffer (Prod. No. ALX-505-005). |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: Schematic structure of human TRAIL (peptide aa 95-281).
Figure: Apoptosis of TRAIL-sensitive cells. Concentration dependence of apoptosis induction in Jurkat and BJAB cells by
KillerTRAIL™, Soluble (human) (recombinant) (Prod. No.
ALX-201-073) reveals high activity even at concentrations of 10-100ng/ml.
Method: 5x10
4 human Jurkat (JK) or BJAB cells were cultured in 100µl RPMI 1640 medium containing 10% fetal calf serum for 16 hours at 37°C in a 96-well plate in the presence of the indicated concentrations of
KillerTRAIL™. Concentrations of
KillerTRAIL™ required to induce apoptosis may vary depending on the cell type studied. Cell death was quantified by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analysed by flow cytometry."
Figure: Flow cytometry analysis of the TRAIL-sensitive human leukemia cell line HL60 and of TRAIL-resistant K572 cells.
Figure: Specificity of
KillerTRAIL™-induced apoptosis. Concentration dependent blocking of apoptosis induction of BJAB cells using MAb to TRAIL (human) (2E5) (Prod. No.
ALX-804-296) reveals specificity of
KillerTRAIL™-mediated killing.
Method: Human BJAB cells were treated with
KillerTRAIL™, Soluble (human) (recombinant) (at 300ng/ml) in the presence or absence of the indicated concentration of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) stain analysed by flow cytometry."
Figure: Apoptosis of TRAIL-sensitive myeloid cell line ML-1.
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Product Literature References
Exploiting the immunogenic potential of standard of care radiation or cisplatin therapy in preclinical models of HPV-associated malignancies: J.T. Kowalczyk, et al.; J. Immunother. Cancer
11, e005752 (2022),
Abstract;
Differences and similarities in TRAIL-and tumor necrosis factor-mediated necroptotic signaling in cancer cells: J. Sosna, et al.; Mol. Cell. Biol.
36, 2626 (2016),
Abstract;
The SMAC mimetic BV6 induces cell death and sensitizes different cell lines to TNF-α and TRAIL-induced apoptosis: M. El-Mesery, et al.; Exp. Biol. Med.
241, 2015 (2016),
Abstract;
Chronic lymphocytic leukemic cells exhibit apoptotic signaling via TRAIL-R1: M. MacFarlane, et al.; Cell Death Differ.
12, 773 (2005),
Abstract;
TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors: M. Plasilova, et al.; Leukemia
16, 67 (2002),
Abstract;
TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Sträter, et al.; Gastroenterology
122, 659 (2002),
Abstract;
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett.
503, 151 (2001),
Abstract;
The cytokines tumor necrosis factor-alpha (TNF-alpha ) and TNF-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papillomavirus-16 E7 oncoprotein: J.R. Basile, et al.; J. Biol. Chem.
276, 22522 (2001),
Abstract;
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